Table I.
List of strains used in this study
| Bacterial Strain | Description/Genotype | Reference |
|---|---|---|
| NTHi 3655 | Clinical isolate from a 10-y-old child with AOM | (36) |
| NTHi 3655ΔompP5 | CmRa. NTHi 3655 with ompP5 gene replaced by a chloramphenicol resistance gene | This study |
| NTHi KR271 | Clinical isolate from blood culture of a 75-y-old individual with bacteremia | (37) |
| NTHi KR271ΔompP5 | CmR. NTHi KR271 with ompP5 gene replaced by a chloramphenicol resistance gene | This study |
| E. coli BL21(DE3) | F-ompT hsdSB (rB- mB-) gal dcm (DE3) | Novagen |
| E. coli::ompP5 3655 | AmpRb. E. coli BL21 (DE3)–bearing recombinant plasmid of pET16b with ompP5 ORF insertion expressing recombinant P5 from NTHi 3655 at the surface. | This study |
| E. coli::ompP5 KR271 | AmpR. E. coli BL21 (DE3)–bearing recombinant plasmid of pET16b with ompP5 ORF insertion expressing recombinant P5 from NTHi KR271 at the surface. | This study |
a
CmR is resistant to chloramphenicol.
b
AmR is resistant to ampicillin.