FIGURE 2.

Tumors from PEP-619WW mice have more immune cell infiltrate. B16-OVA tumor-infiltrating CD45⁺ cells were identified by flow cytometry. (A) Representative flow cytometry plots (lymphocytes > single-cell forward light scatter > single-cell side light scatter > live > CD45⁺) and (B) quantification of CD45⁺ tumor infiltrate, determined by flow cytometry. Density of CD45 cells in tumor (count total CD45/volume postresection tumor) at (C) 14 and (D) 30 d posttumor implant. (E) Representative microscopic images from NanoString GeoMX DSP platform indicating regions of interest within these tumor sections showing CD45 stain (red). Scale bar across top, 2 mm. Zoomed-in images of representative regions of tumors from PEP-WT (i and ii). Scale bar in (i), 1 mm; scale bar in (ii), 2 mm. PEP-619WW (iii and iv). Scale bar in (iii), 1 mm; scale bar in (iv), 1 mm. Flow cytometry data shown are from representative experiment. Flow cytometry experiments were repeated at least three times.