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. Author manuscript; available in PMC: 2022 Aug 11.
Published in final edited form as: J Vis Exp. 2021 Aug 11;(174):10.3791/62839. doi: 10.3791/62839

Figure 2: Peripheral CGRP administration evokes light aversion in bright light in two strains of wildtype mice.

Figure 2:

CD1 and C57/BL6J mice were tested according to the timeline described in Figure 1A. (A) The time CD1 mice spent in the light zone per 5 min interval over 30 min (27,000 lux). Time in light data is shown over time during the test (left panel) and as the average time per 5 min interval for individual mice (right panel). Comparisons were made between vehicle and CGRP at each time point, and between Tx and Pre2 or Post as indicated by brackets. (Veh, n=19; 0.1 mg/kg CGRP, n=19) (B) Time C57BL/6J mice spent in the light zone per 5 min interval over 30 min (27,000 lux). Time in light data are shown over time during the test (left panel) and as the average time per 5 min interval for individual mice (right panel) (Veh, n=42; 0.1 mg/kg CGRP, n=44). (C) The mice from panel B were also analyzed for resting behavior in the dark and light zones during the light/dark assay. (D) The mice from panel B were subsequently tested in the open field assay. The percentage of time spent in the center of the chamber per 5 min interval over 30 min after treatment with vehicle or CGRP (0.1 mg/kg, i.p.) (Veh, n=9; 0.1 mg/kg CGRP, n=9). The percentage of time in the center data is shown over time during the test (left panel) and as the average percentage of the time in the center per 5 min interval for individual mice (right panel). For all panels, mean±SEM is shown, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. This figure is modified from Mason et al. 201710.