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. 1999 Feb;37(2):290–295. doi: 10.1128/jcm.37.2.290-295.1999

FIG. 3.

FIG. 3

Autoradiographs showing semiquantitative RT-PCR analysis of 16S rRNA levels in three strains of M. tuberculosis exposed to either no drug, 0.2 μg of INH per ml, or 1 μg of RIF per ml. (A) Drug-sensitive strain ATCC 27294; (B) INH-resistant strain ATCC 33823; (C) RIF-resistant strain ATCC 35838. Lanes: 1 and 2, time zero, prior to the addition of either INH or RIF; 3, 24 h, no drug; 4, 24 h, INH; 5, 24 h, RIF; 6, 72 h, no drug; 7, 72 h, INH; 8, 72 h, RIF; 9, RT buffer to which target was not added; 10, 5 × 104 genome equivalents of M. bovis ATCC 19210 DNA.