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. 2021 Sep 1;2021:5387799. doi: 10.1155/2021/5387799

Figure 2.

Figure 2

Effect of melatonin on NLRP3 inflammasome-mediated cardiac pyroptosis in MI mice. (a) Representative images from TUNEL staining in different groups. Scale bar, 20 μm. (b) Melatonin treatment decreased the release of LDH in MI mice. ∗P < 0.05 vs. sham, #P < 0.05 vs. MI. Number of trials = 6. (c) The level of GSDMD-N was decreased by melatonin treatment. ∗P < 0.05 vs. sham, #P < 0.05 vs. MI. Number of trials = 5. (d, e) Melatonin treatment reduced the levels of NLRP3 and cl.casp-1 in the hearts of MI mice. ∗P < 0.05 vs. sham, #P < 0.05 vs. MI. Number of trials = 5‐6. (f) The mRNA levels of NLRP3 and caspase-1 in the different groups were detected by real-time PCR. ∗P < 0.05 vs. sham, #P < 0.05 vs. MI. Number of trials = 6. (g, h) The levels of IL-1β and IL-18 in heart tissue from different groups. ∗P < 0.05 vs. sham, #P < 0.05 vs MI. Number of trials = 4‐5. MI: myocardial infarction; cl.casp-1: cleaved caspase-1; MLT: melatonin.