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. 2021 Sep 1;2021:5387799. doi: 10.1155/2021/5387799

Figure 4.

Figure 4

Effect of melatonin on the TLR4/NF-κB signaling pathway both in vivo and in vitro. (a) Melatonin treatment reduced the levels of TLR4 and p65 in the hearts of MI mice. ∗P < 0.05 vs. sham, #P < 0.05 vs. MI. Number of trials = 4. (b) Representative staining of TLR4 by immunohistochemistry. Scale bar, 20 μm. (c) The levels of TLR4 and p65 were decreased in H9C2 cells by melatonin treatment. ∗P < 0.05 vs. ctl, #P < 0.05 vs. hypoxia. Number of trials = 3. (d) TLR4 levels were detected by immunofluorescence. Scale bar, 50 μm. (e) The protein levels of TLR4 and p65 in the hearts of MI mice with or without LPS treatment (2 mg/kg). ∗P < 0.05 vs. sham, #P < 0.05 vs. MI, $P < 0.05 vs. MLT. Number of trials = 4. MLT: melatonin.