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. 2021 Sep 8;218(10):e20200853. doi: 10.1084/jem.20200853

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© 2021 Paillet et al.

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Figure 8. — Some T cell clonotypes enriched in PBC liver also infiltrate CCA lesion. (A–D) Following the experimental schedule illustrated in Fig. 3 A, SB1-JP4 CCA tumor, liver, and blood of a PBC mouse were collected at day 70 after CCA engraftment. T cells of the malignant, hepatic, and blood tissues were sorted out and underwent single-cell transcriptomic analysis (scRNA-seq). Output data were used not only for T cell gene expression profiling but also for TCR sequencing, thus allowing characterization of clonotype. (A) Venn diagram of the T cell clonotype overlap between tumor, liver, and blood. (B) Proportion of T cell clonotypes among total T cells analyzed in each tissue. Clonotypes with a frequency >0.1% were considered enriched. Pairwise P values were calculated using Fisher’s exact test on the percentage of enriched T cell clonotypes. ****, P < 0.0001. (C) Number of enriched T cell clonotypes expanded only in tumor (n = 103), or liver (n = 58), or in both tissues (n = 25). (D) Proportion in the liver versus CCA tumor of each of the 25 T cell clonotypes enriched and shared between both tissues.