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. 2021 Jul 23;40(36):5548–5558. doi: 10.1038/s41388-021-01956-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A mRNA expression levels of lipid mediator receptors were analyzed using the RNA-seq data from the TARGET osteosarcoma project database (n = 101). B mRNA expression levels of lipid mediator receptors were analyzed using the RNA-seq data of bone cancer according to classification of the Cancer Cell Line Encyclopedia. OS, osteosarcoma; ESFT, the Ewing sarcoma family of tumors; CS, chondrosarcoma. C, D mRNA and protein expression levels of LPAR1 in eight human osteosarcoma cell lines. LPAR1 levels were detected by qPCR (C) or immunoblotting (D). All data are shown as means ± SD (n = 3). E Protein expression levels of LPAR1 in 19 tumor samples isolated form osteosarcoma patient-derived xenograft mice.