Fig. 6. Pharmacological inhibition of LPAR1 prevents pulmonary metastasis of osteosarcoma.

A Scheme for the animal study. ONO-7300243 was orally administrated 30–60 min before the intravenous injection of osteosarcoma cells. B, C BLIs of mice intravenously injected HuO9/Akaluc cells. 1 × 10⁶ cells of HuO9/Akaluc cells were intravenously injected into nude mice. Mice were intraperitoneally injected with 100 μL of 5 mM AkaLumine-HCL and underwent BLI. Images were taken 1.5–3 h, 1 day, and 2 days after cell injection (B), and their total flux was calculated by the IVIS imaging system (C). D, E BLIs of mice intravenously injected G-292/Akaluc cells. 1 × 10⁶ cells of G-292/Akaluc cells were intravenously injected into SCID-beige mice. Mice were intraperitoneally injected with 100 μL of 5 mM AkaLumine-HCL and underwent BLI. Images were taken 1.5–3 h, 1 day, 2 days, 7 days, and 14 days after cell injection (D), and their total flux in lung was calculated by the IVIS imaging system (E). **p < 0.01, *p < 0.05 as determined by the Mann-Whitney U test.