FIG. 4.

(Top) Endonuclease restriction map of a cloned genomic DNA fragment of the MHR3 gene. B, BamHI; E, EcoRI; S, SalI; and X, XbaI. The transcription start site (+1) and the DNA (capital letters) and amino acid (titlecase) sequences bordering the first intron are indicated. Sequence data for the MHR3 upstream region are deposited in GenBank (AF086951). (Bottom) S1 nuclease protection assay for MHR3 mRNA. The antisense labeled probe is as indicated in the map. Lanes: 1, yeast RNA; 2, total RNA from GV1 cells treated with 2 μg of 20E per ml for 6 h; 3, total RNA from day 2 4th instar larval epidermis treated with 2 μg of 20E per ml for 21 h; 4, total RNA from GV1 cells. A DNA sequence reaction (lanes G, T, A, and C) was used to indicate the precise sizes of the S1 nuclease-protected fragments.