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GMSAs using the EcRE1 (A), EcRE2 (B), and EcRE3 (C) oligonucleotides and lysate (D) (see Materials and Methods). Cotransfections were as follows: Extract P, empty pIE1hr/PA; Extract B1+U1, pIE1hr–EcR-B1 and pIE1hr–USP-1; Extract B1+U2, pIE1hr-EcR-B1 and pIE1hr-USP-2. Five micrograms of the extracts was used. The antibodies used are indicated above the gels.
