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Competition GMSAs using 100× excess probe DNA used in Fig. 8 (EcRE1 [A], EcRE2 [B], and EcRE3 [C]) and the combination of the extracts, which show that all of the major shifted bands are probe specific. As a nonspecific competitor, 100× MRE DNA (see Materials and Methods) was used. Five micrograms of the extracts was used. See the legend to Fig. 8 for definitions of the abbreviations.
