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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Chromosoma. 2021 Mar 21;130(2-3):177–197. doi: 10.1007/s00412-021-00754-z

Fig.1.

Fig.1

XIST targeting. (a) Schematic map showing the structure of the XIST gene locus corresponding to bases 73,040,491 – 73,072,558 of the human X-chromosome (GRCh37/hg19). The open boxes represent the location and size of exons 1–8, joined by introns. The shaded interval overlapping the 5’ end of exon-1 is expanded in part-b. (b) Schematic map corresponding to the promoter region and 5’ end of the first exon of human XIST, corresponding to 73,071,053 – 73,074,052 of the human X-chromosome (GRCh37/hg19). The location of the gRNA target sites is indicated, as is the location of the oligonucleotides used for PCR across the deleted interval. The thick black horizontal line corresponds to the location of the minimal promoter (Hendrich et al. 1997). The open rectangle represents the 5’ end of XIST exon-1, and the location of the A-repeats is indicated by the shaded area.