Table 2.

Primers used in PCR reactions to introduce either the E3 tag and the signal peptide for increased membrane expression, or the N-terminal Nluc for BRET-based binding assays

Primer	Sequence
E3-tag forward	ATGTGCGAGATCGCCGCCCTGGAGAAGGAGATCGCCGCCCTGGAGAAGGAGATCGCCGCCCTGGAGAAGGGCGGCTCAATGGAAGATTTGGAGGAAACATTATTTG
E3-tag reverse	GCGATCTCGCACATGGTGGCACGCGTGG
SigP forward	ATGCAGCCGCCTCCAAGTCTGTGCGGACGCGCCCTGGTTGCGCTGGTTCTTGCCTGCGGCCTGTCGCGGATCTGGGGATGCGAGATCGCCGCCCTGGAG
SigP reverse	GAGGCGGCTGCATGGTGGCACGCGTGG
Nluc-forward	ATATACGCGTGCCACCATGAACTCCTTCTCCACAAGCGCC
Nluc-linker-reverse	GCTGCCTCCGCCTCCGCTCGCCAGAATGC
linker-GPR1-forward	GGAGGCGGAGGCAGCGAAGATTTGGAGGAAACATTATTTGAAG
GPR1-eYFP-reverse	ATATTCTAGACTACTTGTACAGCTCGTCCATGCCGAG

Parts in bold correspond to the sequences not present in the respective template