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. Author manuscript; available in PMC: 2022 Sep 7.
Published in final edited form as: Cell Metab. 2021 Aug 20;33(9):1853–1868.e11. doi: 10.1016/j.cmet.2021.08.002

Figure 2: sEVs from palmitate-stressed adipocytes induce cardiac ROS in vivo.

Figure 2:

A, Protein carbonylation (PC) assay on cardiac tissue from chow-fed wild-type mice treated 2 hours after injection of sEVs from healthy adipocytes. B, Experimental design for C-H. C, PC determination in heart tissue post sEVPA injection at the indicated timepoints. D, mitoP/B ratio in cardiac tissue 4 hours post sEVPA injection. E, Catalase (CAT) protein expression in whole cardiac tissue 2 hours following a sEVPA injection. F and G, Seahorse Analysis of cardiac mitochondria isolated from mice 1 hour (F) or 2 hours (G) following the indicated injections. Palmitoyl-carnitine and malate were provided as energetic substrates. H, Palmitoyl-carnitine oxidation in isolated cardiac mitochondria 2 hours post sEVPA injection as measured by optical oxygen respirometry. Data are presented as mean ± s.e.m. *P < 0.05, ** P < 0.01, *** P < 0.001. See also Figure S3 and S4.