Skip to main content
. Author manuscript; available in PMC: 2022 Sep 7.
Published in final edited form as: Cell Metab. 2021 Aug 20;33(9):1853–1868.e11. doi: 10.1016/j.cmet.2021.08.002

Figure 4: Mitochondrial-derived vesicles are packaged into sEVs.

Figure 4:

A, Electron micrograph of a mitochondrion displaying a budding structure (left) and released MDVs (right). B, Nanoparticle tracking (NTA) quantification of MDVs released from isolated mitochondria from control and adipo-FtMT sWAT. C and D, VDAC and HSP60 protein content in MDVs released from isolated sWAT mitochondria under the specified conditions. E Immunofluorescent co-stain for TOM20 (mitochondria) and EEA1 (endosomes) with or without antimycin A exposure. F, COXIV content in sEVs isolated from in vitro-differentiated adipocytes treated as indicated (anti-A, antimycin A; CQ, chloroquine). G, Mitochondrial proteins in sEVs isolated from the media of in vitro-differentiated adipocytes of the respective genotypes. sEVs recovered from equal amounts of media were analyzed by Western blot. H, Cardiac PC 1 hour following sEVPA injections from adipocytes with the indicated genotypes. Data are presented as mean ± s.e.m. *P < 0.05, ** P < 0.01, *** P < 0.001.