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. 2021 Aug 16;78(17-18):6337–6349. doi: 10.1007/s00018-021-03910-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Effect of eIF4Ai on anti-IgM-induced MCL1 protein and mRNA expression. CLL samples were pre-treated with silvestrol, rocA or DMSO (solvent control) or left untreated for 1 h and then incubated with anti-IgM for 24 h. Samples were also treated with control antibody. After 24 h expression of (A, B) MCL1 and HSC70 (loading control) was analyzed by immunoblotting. Figure A shows representative immunoblot and (B) quantification of all samples analyzed (n = 4). C, D Expression of MCL1 mRNA was quantified by Q-PCR for c silvestrol (n = 12) or D rocA-treated cells (n = 6). Student’s t tests were performed to determine the statistical significance of differences between test conditions and DMSO/anti-IgM-treated cells (where not shown, P > 0.05). E Polysome profiling of MCL1 mRNA (n = 4). Graphs show values for individual samples and mean (± SD) expression of polysome/monosome (P/M) ratio with values for DMSO/anti-IgM treated cells set to 1.0. A Student’s t test was performed to determine the statistical significance of differences between control antibody and anti-IgM-treated cells