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. Author manuscript; available in PMC: 2022 Sep 9.
Published in final edited form as: J Med Chem. 2021 Aug 20;64(17):13038–13053. doi: 10.1021/acs.jmedchem.1c01130

Figure 3.

Figure 3.

Inhibition of Ras signaling by B4-27. (a, b) Dose-de-pendent inhibition of the interaction between KRasG12V (a) or KRasG12D (b) and CRAF RBD by B4-27 or B3-4 in HEK293T cells as monitored by the BRET2 assay.37 BRET signals reported represent the mean ± SD from 4 independent experiments and are relative to that of vehicle (DMSO)-treated cells (100%). ****, p < 0.0001. (c) Western blots showing the effect of B4-27 on the phosphorylation levels of signaling proteins downstream of Ras (Akt, MEK) in H358 cells. GAPDH was used as a loading control. Representative images from 4 independent experiments are shown.