Skip to main content
. Author manuscript; available in PMC: 2022 Sep 9.
Published in final edited form as: J Med Chem. 2021 Aug 20;64(17):13038–13053. doi: 10.1021/acs.jmedchem.1c01130

Figure 4.

Figure 4.

B4-27 causes apoptotic death of Ras mutant cancer cell lines. (a) Effect of B4-27 on the viability of various cancer and non-cancerous cell lines as monitored by the Cell-Titer Glo® 2.0 Viability Assay. Cells were incubated with indicated concentrations of B4-27 for 96 h in the presence of 10% FBS. (b) Annexin V/PI staining of H358 cells after treatment with indicated concentrations of B4-27 for 24 h in the presence of 10% FBS. Cytometry data were gated so that Q1 contained necrotic cells, Q2 contained late apoptotic cells, Q3 represented early apoptotic cells and Q4 were live cells. (c) Percentage of annexin V-positive cells (in Q2 and Q3) as a function of B4-27 concentration. Data shown represent the mean ± SD from at least three independent experiments.