Table 1.
Scheduled multiple reaction monitoring parameters
| precursorion | production 1 | production 2 | production 3 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGE/amino acid internal standard | RT | m/z | DP | m/z | CE | CXP | m/z | CE | CXP | m/z | CE | CXP |
| OH-Pro | 0.84 | 132.0 | 38 | 86.0 | 19.1 | 6.9.0 | 68.0 | 26.1 | 5.1 | 57.9 | 36.7 | 9.1 |
| Glucosepane | 6.67* 6.84 |
429.3 | 20 | 384.5 | 38.0 | 19.0 | 269.2 | 55.0 | 20.0 | 339.2 | 55.0 | 20.0 |
| GOLD | 6.68 | 327.2 | 60 | 84.1 | 55.0 | 13.0 | 282.3 | 29.0 | 11.0 | 198.1 | 25.0 | 19.0 |
| MODIC | 7.10 | 357.3 | 25 | 312.2 | 31.0 | 14.0 | 267.3 | 45.0 | 15.0 | 197.4 | 45.0 | 14.0 |
| GALA | 2.36 | 205.2 | 40 | 142.1 | 19.0 | 10.0 | 84.1 | 25.0 | 13.0 | 159.1 | 15.0 | 13.0 |
| MOLD | 7.10 | 341.3 | 45 | 296.3 | 33.0 | 18.0 | 84.2 | 52.0 | 14.0 | 212.3 | 29.0 | 21.0 |
| MG-H3 | 6.00 | 229.2 | 45 | 114.1 | 22.5 | 9.0 | 70.1 | 45.0 | 14.0 | 116.1 | 21.0 | 9.0 |
| MG-H1 | 6.00 | 229.2 | 55 | 70.1 | 43.0 | 12.0 | 116.1 | 20.5 | 9.0 | 114.1 | 22.5 | 9.0 |
| CMA | 4.89 | 233.1 | 45 | 70.1 | 45.0 | 15.0 | 116.1 | 23.0 | 10.0 | 118.2 | 22.0 | 5.5 |
| Pyrraline | 6.81 | 255.2 | 38 | 175.2 | 17.0 | 14.0 | 237.2 | 12.0 | 11.0 | 148.3 | 25.0 | 10.0 |
| DT-Ha | 5.47 | 259.1 | 45 | 144.1 | 22.0 | 9.0 | 70.0 | 45.0 | 10.0 | 116.1 | 25.0 | 17.0 |
| CEA | 5.88 | 247.1 | 51 | 70.2 | 48.0 | 12.0 | 116.2 | 25.0 | 10.0 | 132.1 | 24.0 | 10.0 |
| CML | 2.36 | 205.1 | 40 | 130.2 | 17.0 | 11.0 | 84.1 | 25.0 | 13.0 | 56.1 | 50.0 | 10.0 |
| CEL | 4.24 | 219.1 | 54 | 84.1 | 33.0 | 11.0 | 130.1 | 18.0 | 12.0 | 56.1 | 59.0 | 8.0 |
RT: retention time (min), m/z: mass-to-charge ratio (atomic mass unit), DP: declustering potential (V), CE: collision energy (eV), CXP: cell exit potential (V)
*
The two isomer peaks with different retention times were integrated together and the sum was used.