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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Biomed Pharmacother. 2021 Jul 15;141:111912. doi: 10.1016/j.biopha.2021.111912

Figure 2. Treprostinil reduces renal tubular apoptosis.

Figure 2.

(A) Renal apoptosis in situ, measured by TUNEL staining (red fluorescence) of outer medullary region in rat kidney sections at 6-hour post-reperfusion (×400, scale bar = 50 μm). Nuclei were counterstained with Hoechst (blue fluorescence). Random fields (n=4–5/section) with the same acquisition setting were examined for each slide. (B) Apoptosis was determined by the intensity of TUNEL/Hoechst, normalized to the area and quantified with the same threshold using image J software. (C) Renal cytosol cytochrome c protein levels at 1-hour post-reperfusion by western blot, normalized to Gapdh, and quantification was performed using image J software. Data are represented as mean ± SD; *p<0.05, **p<0.01 vs. sham, †p<0.05, ††p<0.01 vs. placebo (n = 3–5/group). One-way ANOVA, Tukey’s multiple comparison test. TUNEL: Terminal deoxynucleotidyl transferase dUTP nick end labeling; cyto: cytosol; mito: mitochondria.