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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Biomed Pharmacother. 2021 Jul 15;141:111912. doi: 10.1016/j.biopha.2021.111912

Figure 5. Treprostinil preserves mtDNA and mitochondrial biogenesis.

Figure 5.

(A) Renal mtDNA copy number at 1, 3, and 6 hours after reperfusion determined by the ratio of mt-Nd1/Gapdh, measured by real-time qPCR; (B) Renal mRNA expression of Pgc-1α at 1-, 6-, 24-, and 48-hours post-reperfusion was measured by real-time qPCR, normalized to Gapdh and is expressed as a fold-increase over sham kidney. Data are represented as mean ± SD; *p<0.05 **p<0.01, ***p<0.001 vs. sham, †p<0.05, ††p<0.01, †††p<0.001 vs. placebo (n = 4–5/group). Two-way ANOVA, Tukey’s multiple comparison test. mtDNA: mitochondrial DNA; mt-Nd1: mitochondrially encoded NADH dehydrogenase 1; Pgc-1α: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha.