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. 2021 Sep 9;11:17946. doi: 10.1038/s41598-021-97073-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence imaging of live cells. (a) Fluorescence live cell imaging of hepatocellular carcinoma (HCC) cell lines (Hep-G2, HuH-7, PLC/PRF/5, and Li-7) and HUVECs using SPiDER-βGal (1 μM). Fluorescence was determined using a TRITC filter (left) and Brightfield images (right); images were captured using a Keyence BZ-X800 with a TRITC filter (Excitation: 545/25 nm, Emission: 605/70 nm, Exposure time: 2 s). Scale bar = 100 μm. (b) The average fluorescence intensity of 10 randomly selected live cells imaged with SPiDER-βGal was determined using ImageJ (n = 10). Fluorescence intensity was significantly higher in HCC cell lines than in HUVECs (**p < 0.01). A two-tailed Mann–Whitney U-test was used. Error bars represent standard error (SE) values.