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. 2021 Sep 9;11:17946. doi: 10.1038/s41598-021-97073-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence imaging of tumours in four tumour-bearing mouse models. (a) Fluorescence images of tumours and normal livers were captured 0, 10, and 30 min after spraying SPiDER-βGal (50 μM) using IVIS Lumina Series III (Excitation: 520 nm, Emission: 570 nm). White light images are also shown. Left: normal liver, Right: tumour. Scale bar = 10 mm. (b) Average fluorescence intensity of tumours and normal livers resected from tumour-bearing mice (Hep-G2, n = 3; HuH-7, n = 4; PLC/PRF/5, n = 4; Li-7, n = 4). After 30 min of spraying, HuH-7 tumours, PLC/PRF/5 tumours, and Li-7 tumours had a significantly higher fluorescence intensity than normal liver tissues (*p < 0.05). A two-tailed Mann–Whitney U-test was used. Error bars represent standard error (SE) values.