Fig. 3. Pre-exposure to NaCl desensitizes ASEL.

a, b Animals were pre-exposed to 100 mM NaCl for 60–600 s, washed briefly (60 s), and exposed to 100 mM NaCl. a Average Ca²⁺ transient (±SEM) in ASEL in response to 100 mM NaCl after pre-exposure for 60 s (n = 14), 120 s (n = 9), 300 s (n = 8), or 600 s (n = 16 animals). b Average maximum ratio changes (±SEM) in ASEL after pre-exposure: responses after 300 and 600 s pre-exposure were significantly reduced, compared to responses after 60 or 120 s pre-exposure. c, d Animals were pre-exposed to 100 mM NaCl for 10 min, before test exposure to 200, 300, or 400 mM NaCl. c The average Ca²⁺ transients (±SEM) and (d) the average maximum ratio changes (±SEM) in ASEL. Exposure to 300 (n = 10 animals) or 400 mM (n = 7) NaCl yielded significantly stronger Ca²⁺ transients than 200 mM (n = 8). e, f Animals were pre-exposed to 100 mM NaCl for 10 min, washed for 1, 2, or 5 min in a NaCl-free buffer and re-exposed to 100 mM NaCl, where (e) shows the average Ca²⁺ transients (±SEM) and (f) the average maximum ratio changes (±SEM) in ASEL. Five minutes wash with a NaCl-free buffer restored the Ca²⁺ response of ASEL to 100 mM NaCl. Wash time: 1 min (n = 16), 2 min (n = 8), 5 min (n = 6 animals). Individual data points have been indicated as dots. Statistically significant differences have been indicated (non-significant differences, p > 0.05, have not been indicated). Source data underlying this figure are available in Supplementary Data 3.