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. 2021 Sep 9;12:5341. doi: 10.1038/s41467-021-24894-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Heatmap views for RING1B and SUZ12 distribution at the TSS (5 kb upstream to 5 kb downstream) of each gene in WT (fl/fl) or Pcgf1-KO (∆/∆) ESCs, with or without (DMSO) Triptolide treatment. In parallel, the distribution of PCGF1 in untreated control ESCs, and H2AK119ub1 enrichment in control (fl/fl) or Pcgf1-KO (∆/∆) ESCs without Triptolide treatment, are shown as a reference. BioCAP data (SRR648805 [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1064680], deposited in GEO) showing the distribution of unmethylated CpGs are also shown. Genes were sorted into six groups by k-means clustering (n = 6) based on the distribution of RING1B, SUZ12, and PCGF1 in WT (fl/fl) ESCs. The number of genes in each cluster is shown within parentheses. b Violin plot showing differential gene expression in each cluster in untreated ESCs. Significant differences were calculated by Mann–Whitney U test (one-sided). The p-values are adjusted by BH methods. c Average binding of RING1B and SUZ12 in Cluster 4 genes in WT or Pcgf1-KO ESCs and EBs. Metaplot views of RING1B and SUZ12 binding at the TSS (5 kb upstream to 5 kb downstream) of Cluster 4 genes, in WTl (fl/fl) or Pcgf1-KO (∆/∆) ESCs, with or without triptolide treatment. Data for triptolide-treated, or untreated, ESCs are shown by purple and green lines, respectively. d Distribution of RING1B and SUZ12 at selected Group 1 genes (Klf4, Tbx3, Pdgfa) and Group 3 genes (Sox4, Grhl2) in control ESCs with or without (DMSO) Triptolide treatment. Genomic snapshots of RING1B and SUZ12 distributions in WT (fl/fl) or Pcgf1-KO (∆/∆) ESCs with or without (DMSO) triptolide treatment are shown.