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. 2021 Aug 11;18(9):2188–2198. doi: 10.1038/s41423-021-00749-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Characterization of clinical-grade MPTK-CAR-T cell products. A Flow cytometric analysis of CAR, PD-1, and TCR surface expression in MPTK-CAR-T cell products. Individual data points and the mean with SD are shown. B The frequencies of gene editing for PDCD1 and TRAC in 9 MPTK-CAR-T infusion products. Individual data points and the mean with SD are shown. C Translocation frequency in 15 MPTK-CAR-T cell products. T1 to T4 represent four different possible translocation events. D CD8/CD4 ratio of MPTK-CAR-T cell products. E In vitro expansion of MPTK-CAR-T cell products. The fold changes in the cell number of MPTK-CAR-T cells after electroporation are shown. F Cytotoxicity assay using MPTK-CAR-T cells and normal T cells as effectors and the CRL5826-PD-L1 cell line as a target. Individual data points and the mean with SD at an E:T ratio of 1:1 are shown. MPTK-CAR-T cells vs. normal T cells, ****P < 0.0001. G IFN-γ release assay using MPTK-CAR-T cells and normal T cells as effectors and the CRL5826-PD-L1 cell line as a target. Individual data points and the mean with SD at an E:T ratio of 1:1 are shown. MPTK-CAR-T cells vs. normal T cells, ****P < 0.0001. Statistics were analyzed with an unpaired t test (F and G)