Fig. 4. The peripherally restricted oxytocin receptor antagonist, Atosiban, does not reverse chemogenetic-induced reduction in alcohol drinking.

A In mice harboring the excitatory DREADD, CNO-induced activation of PVN^OT+ neurons decreased alcohol consumption compared to vehicle. Pretreatment with Atosiban (1 mg/kg) did not affect alcohol consumption in vehicle (Saline + Atosiban) or CNO (CNO + Atisoban) groups. B Neither CNO nor Atisoban pretreatment altered alcohol drinking in animals treated with control virus. Values are mean ± s.e.m. (N = 5–6/group); *p < 0.05 vs. saline.