Fig. 6.

CircINPP4B regulated Th17 cell differentiation via miR-30a. A–D CD4⁺ Tn cells were infected with miR-NC, miR-30a, wt-circINPP4B or mut-circINPP4B before induction of Th17 polarization. Three days later, flow cytometric analysis of the stimulated IL-17⁺ cells was performed and is shown in (A and B). ELISAs of IL-17A, IL-17F, IL-21, TNF-α and GM-CSF in culture supernatants is presented in (C). RT-qPCR analysis of Rorγt and IL-21R expression in the cultured cells is displayed in (D). E, F CD4⁺ Tn cells were infected with negative control (NC) or circINPP4B siRNA and then induced under Th17 polarizing conditions with or without IL-21. Three days later, IL-17⁺ cells in the CD4⁺ gate were analyzed by flow cytometry. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001