Table 4.
Primers used for the qPCR analysis of the target candidate genes for FHS in the femur articular cartilage of broilers.
| Gene | Ensembl ID | Primer sequences (5'-3') |
|---|---|---|
| AvBD1 | ENSGALG00000022815 | F: CAGGATCCTCCCAGGCTCTA |
| avian beta-defensin 1 | R: GATGAGAGTGAGGGAAGGGC | |
| AvBD2 | ENSGALG00000016669 | F: TTCTCCAGGGTTGTCTTCGC |
| avian beta-defensin 2 | R: TGCATTCCAAGGCCATTTGC | |
| FBN2 | ENSGALG00000014686 | F: TGCATCGATAGCCTGAAGGG |
| fibrillin 2 | R: CTAATTCACACCGCTCACATGG | |
| ANK1 | ENSG00000029534 | F: CCACCATCCCACCATTCAGT |
| Ankyrin 1 | R: ACGGTCACAAACTCCAGCAT | |
| PSPH | ENSGALG00000002397 | F:CAGGAATACGGGAGCTGGTG |
| phosphoserine phosphatase | R: CCCAGAGACCAGGAAGACCT | |
| EPX | ENSGALG00000043254 | F:AAAGGAGGTGGCATTGACCC |
| eosinophil peroxidase | R: GCCACGCTGCATGTTAAGAG | |
| ADA | ENSGALG00000004170 | F:TTCGGCAAGAAAAGAGGGGT |
| adenosine deaminase | R: GTGTTTGGTAGCTGACGTGC | |
| COL13A1 | ENSGALG00000004286 | F:CCAAGCAAGGACTAGACACTCA |
| collagen type XIII alpha 1 chain | R: ACCCTTCATGCCATGTCTTCC | |
| CSF3R | ENSGALG00000002112 | F: TCATCCGGGACAGCATTGAG |
| colony stimulating factor 3 receptor | R: TGTAGAGGGGGTACACCGAG | |
| RHAG | ENSGALG00000016684 | F:TCTGGAGATCACGGCCTTTG |
| Rh associated glycoprotein | R:GCTCCAATATCTGTGGCCTGA | |
| S100A9 | ENSGALG00000024272 | F:GGGGACAAAGACACCCTGAC |
| S100 calcium binding protein A9 | R:TTCACGTGCTTCAGGTAGTTGG |