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. 2021 Sep 9;11:18000. doi: 10.1038/s41598-021-97339-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibitory effects of human serum against direct NS1 detection. Inhibitory effects of different human serum samples were analyzed using the aptamer/antibody (Apt/Ab) ELISA format. Each UB-DNA aptamer was employed as the capture agent, and the antibody, Ab#D06, was used as the primary detector agent. Each serotype DEN-NS1 was added in buffer (a) or human serum purchased from Sigma (untreated in b or treated with protein A resin for removal of IgGs in c), or each human serum sample obtained from three different persons with no current dengue infection (d–f). The mixtures were subjected to ELISA at the final 10% human serum concentration. A certain amount of the recombinant DEN-NS1 purchased from The Native Antigen Company (350 pg for DEN1-NS1, 350 pg for DEN2-NS1, 450 pg for DEN3-NS1, and 200 pg for DEN4-NS1) was added in each well (50 μL).