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. 2021 Sep 9;11:18000. doi: 10.1038/s41598-021-97339-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Serotype-specific anti-DENV-NS1 IgG detection using convalescent-phase ZIKV clinical samples by the competitive Apt/Ab ELISA format. For the competitive ELISA tests, 5 µL of diluted plasma samples (corresponding to the use of 0.0005–5 µL) were used and the amounts of the spiked DEN-NS1 were adjusted to obtain similar signal intensities, as shown in Supplementary Fig. 1. ZIKV IgG detection patterns in each acute-phase sample are from the ELISA results reported previously⁷⁴ and Dengue IgG detection patterns are from the tests using the SD BIOLINE Dengue Duo kit (Dengue IgG/IgM test). n.d. not determined (not tested.).