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. 2021 Sep 9;7:74. doi: 10.1038/s41522-021-00246-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The saliva inoculum was mixed with 0.7% agar containing biofilm growth media—either BHI (a) or TP (b), and inoculated onto one of the substrata (RHG, HAP or TI). The biofilms were collected for viable cell counts on day 1, 3 and 5. RHG: reconstructed human gingiva; HAP: hydroxyapatite discs; TI: titanium discs. Data represent the mean ± standard deviation of three independent experiments. * represents statistically significant difference when compared to the day 1 biofilms (p < 0.05). The dotted line indicates the averaged viable cell counts of saliva inocula of all experiments.