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. 2021 Sep 9;7:74. doi: 10.1038/s41522-021-00246-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Relative abundance of bacterial genera in microcosm biofilms. In total, 13 genera that are found at a relative abundance higher than 1% in any of the samples, were included and counted. The remaining genera are grouped as others. b The Shannon diversity index of microcosm biofilms grown in either BHI (left) or TP (right). The dotted line indicates the Shannon index of the saliva inoculum. * represents statistically significant difference when compared to the day 1 biofilms for each condition (p < 0.05). c Bray-Curtis similarity index between the saliva inoculum and the biofilms grown in either BHI (left) or TP (right) medium for 1, 3 and 5 days. * represents statistically significant difference when compared to the sample pair of day 1 biofilms vs. inoculum (p < 0.05). INO, saliva inoculum; RHG: Reconstructed Human Gingiva; HAP: hydroxyapatite discs; TI: titanium discs. TP: Thompson medium. Data represent the average of 3 independent experiments, each performed in replicates.