FIG. 3.

Binding of different CDP domains to SATB1. GST-CDP (CR2-Cterm), GST-CR1, GST-CR2, GST-CR3, GST-HD, GST-CR3 plus HD, GST-coiled-coil (GST-CC), or GST only (11) (lanes 2 to 9) were incubated with in vitro-translated labeled SATB1 (lane 1), as described for Fig. 2A. As controls, labeled in vitro-translated CDP containing the spacer region between CR1 and CR2 (lanes 10 and 11) or firefly luciferase (lanes 12 and 13) also was incubated with a GST–full-length SATB1 fusion (lane 11) or a GST-CDP (CR2-Cterm) fusion (lane 13). Lanes 1, 10, and 12 show the input radioactively labeled protein.