FIG. 5.

Far-Western blotting to detect SATB1-CDP association. Crude nuclear lysates from murine thymus (lanes 1 and 2) and mink lung cells (lanes 3 to 6) were prepared as described previously (32), resolved on SDS–8% polyacrylamide gels, and stained with Coomassie brilliant blue (lane 1) or electrotransferred to nitrocellulose (lanes 2 to 6). ³⁵S-labeled in vitro-translated CDP was incubated in the absence of competitor protein (lanes 2, 3, and 5) or in the presence of purified recombinant SATB1 (lane 4). Mink lung cell proteins on the membrane also were incubated with an in vitro translation reaction mixture containing [³⁵S]methionine, but no DNA template (lane 6). Lanes 1 to 4 are derived from a single gel; lanes 5 and 6 are from a second gel. The arrows show the positions of SATB1 protein detected by interaction with labeled CDP.