FIG. 7.

CDP overexpression leads to reduced SATB1 DNA binding. (A) Overexpression of CDP determined by Western blotting. Nuclear extracts were prepared 48 h following transfection and used for Western analysis with anti-SATB1 or -CDP serum. Equal amounts of extract were loaded in each lane, and the labeled bands were quantitated by densitometry. (B) CDP overexpression prevents SATB1 DNA binding to the MMTV LTR. The same nuclear extracts as used for panel A were used for gel shift assays with the labeled 120-bp proximal NRE probe (32). The upper band observed in lanes 2 and 3 does not contain CDP, as determined with anti-CDP serum in gel shift assays (not shown). This band may represent binding of two molecules of SATB1 to the 120-bp NRE probe.