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. 2021 Sep 9;12:5330. doi: 10.1038/s41467-021-25630-3

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© The Author(s) 2021, corrected publication 2021

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Fig. 3 — a Analysis by RT-PCR followed by pyrosequencing of Acyp2 and Bag3 allelic expression ratio in 18 clones generated from the F1-21.6 ESC line (left panels). Comparison of the cumulative distributions of allelic expression ratios in ESCs and NPCs (Kolmogorov–Smirnov tests, right panels). n is the number of clones analyzed. b Comparison of the allelic expression ratios of the genes previously categorised as RME in NPCs¹¹ and after differentiation into astrocytes, for five NPC clones, measured by allele-specific RNA-seq. The number of assessable genes per clone (values with less than 20 reads were excluded) is n_XX1 = 75, n_XX2 = 146, n_XX3 = 121, n_XY1 = 150, n_XY2 = 137. Pearson correlation coefficients were calculated for each NPC clone. c Comparison of the allelic expression ratio for the nine genes, which showed several modalities of expression, in five NPC clones and after differentiation into astrocytes.