Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 9;11:17912. doi: 10.1038/s41598-021-97105-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Retrogradely labelled and tdTomato-positive lamina I neurons in horizontal sections from the L3 segment. (a) Part of lamina I from experiment #1, in which cholera toxin B subunit (CTb) was injected bilaterally into the lateral parabrachial area. TdTom and CTb are revealed in magenta and green, respectively. Many double-labelled cells are visible, as well as some that are CTb-positive but lack tdTomato. The box shows the area corresponding to (b–d). (b–d) Show this region at higher magnification, with NK1r-immunoreactivity in blue, tdTom in red and CTb in green. Several retrogradely labelled tdTom-positive cells are visible in this field. The three marked cells are either strongly NK1r-immunoreactive (arrow), weakly NK1r-immunoreactive (arrowhead) or NK1r-negative (double arrow). The image in (a) is projected from a confocal scan through the thickness of the section, while those in (b–d) are projected from 2 optical sections (1 μm z-separation in each case). Scale bars: (a) = 100 μm, (b–d) = 50 μm. (e) Box and whisker plots showing the proportions of Phox2a-positive and -negative cells that were classed as negative (0), weakly-stained (1) or strongly stained (2) for NK1r in experiments #1–4 and #6. Significance is shown with asterisks (**p < 0.01; ****p < 0.0001). (f) Frequency histogram showing the soma sizes of retrogradely labelled neuron analysed in experiments #1–#6. Phox2a-positive and negative cells are represented in magenta and green, respectively, and areas of overlap are shown in grey.