Fig. 2. Cryo-EM structure of CP-bound barbed ends.

A Overview of the Cryo-EM map of capped F-actin. The density has been sharpened and denoised using deepEMhancer⁸² B Atomic model of CP bound to the barbed end. While the penultimate protomer interacts extensively with CP, the barbed end side of the terminal protomer is bound mostly by the β tentacle. We highlight the position of the bound small molecules. Actin subunits are labeled A₀ to A₃ starting from the terminal actin protomer. C The structure of WASP-bound G-actin⁵¹ (PDBID:2A3Z) was superimposed onto the terminal protomer of the complex. The β tentacle occupies the binding pocket that a WH2 domain would need to bind the terminal protomer. Actin monomers are shown as surface, while the tentacle and the WH2 helices are shown as cartoons.