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. 2021 Aug 27;12:666558. doi: 10.3389/fmicb.2021.666558

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Copyright © 2021 Roy Chowdhury, Golas, Alteio, Stevens, Billings, Blanchard, Melillo and DeAngelis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Taxonomic classifications – community composition of heated and control samples using taxonomic assignments from both rRNA and mRNA reads in Organic horizon. “+” indicates enrichment and “*” depletion of phylum in heated compared to control plots. rRNA and mRNA reads were annotated using MATAM and MEGAN programs, respectively. Composition is computed using relative abundance and all assignments are given at the phylum level besides those for proteobacteria, which are at the class level. Note that assignments to eukaryotic phyla occur only for mRNA (as rRNA assignments are performed based on alignments for the 16S sequence, which occurs only in prokaryotes). Only phyla/classes occurring at >3% on average are shown and are otherwise grouped into “Other.” Only for rRNA assignments did phyla in Archaea exceed the 3% threshold for being graphed; no Archaea cleared 0.1% abundance in the mRNA samples.