Table 1.
Sequencing output – RNA sequencing results with percentages of rRNA, mRNA, and number of internal standard reads recovered from each sample.
| Site | Time points | Treatments | Soil types | Replicates | Total reads | rRNA (%) | mRNA reads | mRNA (%) | Internal standard reads |
|---|---|---|---|---|---|---|---|---|---|
| Prospect Hill | T2 (3rd June) | Heated | Organic | 1 | 122,445,353 | 92.37 | 776,829 | 0.63 | 40,167 (0.033) |
| 2 | 119,068,820 | 95.49 | 874,119 | 0.73 | 27,342 (0.023) | ||||
| 3 | 121,681,637 | 91.97 | 1,062,698 | 0.87 | 37,597 (0.031) | ||||
| 4 | 110,749,650 | 93.5 | 1,004,683 | 0.91 | 27,443 (0.025) | ||||
| Mineral | 1 | 200,636,302 | 93.87 | 1,600,486 | 0.80 | 37,076 (0.0001) | |||
| 2 | 106,671,365 | 86.34 | 751,973 | 0.70 | 148 (0.000) | ||||
| 3 | 150,911,448 | 93.17 | 1,429,743 | 0.95 | 11,675 (0.078) | ||||
| 4 | 14,366,071 | 95.32 | 100,969 | 0.70 | 799 (0.006) | ||||
| Control | Organic | 1 | 174,072,328 | 95.01 | 1,266,671 | 0.73 | 21,913 (0.013) | ||
| 2 | 84,722,020 | 87.73 | 2,720,341 | 3.21 | 36,926 (0.043) | ||||
| 3 | 96,103,877 | 93.01 | 834,364 | 0.87 | 37,048 (0.039) | ||||
| 4 | 103,877,062 | 92.61 | 819,071 | 0.79 | 3,577 (0.003) | ||||
| Mineral | 1 | 118,169,425 | 78.73 | 1,650,805 | 1.40 | 135 (0.000) | |||
| 2 | 126,980,438 | 92.86 | 1,026,399 | 0.81 | 32,620 (0.026) | ||||
| 3 | 117,483,686 | 88.15 | 1,608,598 | 1.37 | 341 (0.000) | ||||
| 4 | 51,771,336 | 18.32 | 12,438,133 | 24.03 | 1,661 (0.003) | ||||
| T6 (28th October) | Heated | Organic | 1 | 88,248,858 | 88.54 | 1,013,469 | 1.15 | 1804 (0.002) | |
| 2 | 74,353,265 | 92.62 | 508,318 | 0.68 | 13,097 (0.018) | ||||
| 3 | 115,840,435 | 85.7 | 1,206,587 | 0.01 | 12,966 (0.011) | ||||
| 4 | 113,836,625 | 93.63 | 1,028,287 | 0.90 | 30,889 (0.027) | ||||
| Mineral | 1 | 101,565,281 | 71.70 | 1,523,539 | 1.50 | 79 (0.00) | |||
| 2 | 49,534,972 | 93.07 | 290,162 | 0.59 | 5,536 (0.011) | ||||
| 3 | 121,535,724 | 93.15 | 1,405,430 | 1.16 | 3,405 (0.003) | ||||
| 4 | 102,602,888 | 93.93 | 775,689 | 0.76 | 1911 (0.001) | ||||
| Control | Organic | 1 | 193,899,095 | 93.39 | 1,673,111 | 0.86 | 25,323 (0.013) | ||
| 2 | 131,247,689 | 92.39 | 2,352,189 | 1.79 | 63,261 (0.048) | ||||
| 3 | 49,240,403 | 93.07 | 297,382 | 0.60 | 7,448 (0.015) | ||||
| 4 | 119,163,187 | 94.39 | 967,338 | 0.81 | 32,103 (0.027) | ||||
| Mineral | 1 | 93,916,386 | 82.69 | 1,498,979 | 1.60 | 75 (0.00) | |||
| 2 | 153,163,875 | 94.59 | 1,130,284 | 7.38 | 30,679 (0.020) | ||||
| 3 | 142,257,923 | 95.44 | 877,431 | 0.62 | 11,868 (0.008) | ||||
| 4 | 96,556,916 | 93.40 | 816,565 | 0.85 | 8,982 (0.009) |
In the internal standard read column, values within () indicates % of internal standards sequence to the total number of putative mRNA transcripts.