Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 27;12:742862. doi: 10.3389/fphar.2021.742862

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Gawali, Chimote, Newton, Feria-Garzón, Chirra, Janssen, Wise-Draper and Conforti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

αPD-1 increases Ca²⁺ fluxes in PBTs of a subset of HNSCC patients. (A,B) Representative intracellular Ca²⁺ recordings in activated CD8⁺ PBTs of HNSCC patients are shown on the left side. Cells loaded with Fura-2 were perfused with thapsigargin (TG) in 0 mM Ca²⁺. Perfusion with 0.5 mM Ca²⁺ yields a rapid influx of Ca²⁺ (See Material and Methods). Two types of Ca²⁺ responses to αPD-1 were observed. A significant increase in Ca²⁺ after αPD-1 (10 μg/ml for 6 h) was defined as positive response, PR (A, left) while no change in Ca²⁺ response was defined as no-response, NR (B, left). The subset of HNSCC patients (n = 3/9) showing positive Ca²⁺ response are reported in panel (A, right) while patients (n = 6/9) with no-response are shown in panel (B, right). The corresponding single cell ∆Ca²⁺ values (peak minus baseline before peak) measured in the absence and presence of αPD-1 of PR and NR are shown in the right panels (n = 82–99 cells from 3 patients in PR and n = 140–149 cells from 6 patients in NR). (C) Comparison of ∆Ca²⁺ values of activated CD8⁺ PBTs from HDs n = 376 cells from nine donors and PR (n = 82 cells from 3 patients) and NR (n = 146 cells from 6 patients) HNSCC patients at baseline (before αPD-1). The ∆Ca²⁺ values in panels (A,B,C) are represented as box and whisker plots. The lower and upper bound of the box represent 25^th and 75^th percentiles respectively. Median values are shown as horizontal line in the box. The lower and upper error represents 10^th and 90^th percentile respectively. (D) Relationship between ∆Ca²⁺ values before αPD-1 treatment and fold increase in ∆Ca²⁺ after treatment with αPD-1 antibody in CD8⁺ PBTs of HNSCC patients (n = 9) are represented as correlation analysis. Individual patients from PR and NR group are marked in blue (PR) and black (NR). Data for panel (A) were analyzed using unpaired student’s t-test. Data for panel (B) were analyzed using Mann-Whitney rank-sum test. Data in panel C were analyzed using ANOVA (p < 0.001) followed by Dunn’s test and data in panel D were analyzed using linear regression.