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. 2021 Aug 27;9:735203. doi: 10.3389/fcell.2021.735203

FIGURE 1.

FIGURE 1

PAX2 expression in bipotential supporting cells before sex differentiation. (A) PAX2 and DMRT1 protein expression in E4.5, E5.0, E5.5, and E6.0 male and female chicken gonads. Dotted white line denotes the gonadal mesenchyme versus epithelium. Ms indicates the mesonephros. (B) PAX2 (magenta), DMRT1 (cyan), and cytokeratin (CK, white) immunofluorescence in E5.0 chicken urogenital system. Dashed white box indicates the magnification area; dotted white line denotes the gonadal mesenchyme versus epithelium. Yellow arrows show cells expressing both DMRT1 and PAX2 at high levels; yellow arrowheads indicate DMRT1+ cells expressing low levels of PAX2; brown arrowheads indicate DMRT1 positive PAX2 negative cells. (C) Decline in PAX2 mRNA expression during gonadal sex differentiation. PAX2 mRNA expression by qRT-PCR in E4.5, E6.5, and E8.5 male and female gonads. Expression level is relative to β-actin and normalized to E4.5 male. Bars represent Mean ± SEM. * and ** = adjusted p value < 0.05 and <0.01, respectively. 2-way ANOVA and Tukey’s post-test.