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. 2021 Aug 27;9:735203. doi: 10.3389/fcell.2021.735203

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Copyright © 2021 Estermann, Mariette, Moreau, Combes and Smith.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PAX2⁺ mesenchymal origin of supporting cells is conserved in ratites (emu). (A) PAX2, DMRT1, and SOX9 protein expression in E9.5, E11.5, and E13.5 male emu gonads. (B) PAX2, FOXL2, and DMRT1 protein expression in E9.5, E11.5, and E13.5 female emu gonads. Dashed white line indicates the gonadal epithelium vs. medullary mesenchyme. Ms indicates the mesonephros. DAPI was used as counterstain.