Figure 2.

Rmrp regulated cell proliferation and fibrosis in MC. (A) cells proliferating were performed by EdU after transfection of Rmrp siRNA in HMC, or over-expression Rmrp in LMC (Scale bar, 100 μm). (B) Cell cycle analysis was performed using flow cytometry in MC transfected with si-Rmrp and Rmrp (+). (C) The expressions of cell-cycle-related proteins Cyclin D1 and Cyclin-dependent kinase 4 (CDK4) regulated by Rmrp were analyzed by western blot. (D) The fibrosis-related protein expression of Collagen IV (Col-IV) and Fibronectin (FN) in LMCs transfected with Rmrp (+) and in HMC transfected with siRmrp was analyzed by western blot. Data were represented as the mean ± SD of three independent experiments; *p < 0.05, **p < 0.01, and ***p < 0.001.