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. 2021 Jun 11;8:368–380. doi: 10.1016/j.bioactmat.2021.06.003

Fig. 4.

Fig. 4

Antioxidant effects of Cur-TFNAs via regulating the Nrf2 signaling pathway. (a) Immunofluorescence images of ROS in RAW264.7 cells (ROS: green). Scale bars are 100 μm. (b) Immunofluorescence micrographs of LPS-treated RAW264.7 cells (cytoskeleton: green; nucleus: blue; HO-1: red; 3D-reconstruction: 3D reconstruction of fluorescence microscopic images based on fluorescence intensity of HO-1). Scale bars are 25 μm. (c) The SOD level of RAW264.7 cells were treated with TFNAs, Cur, Cur-TFNAs, and LPS (n = 3). (d) Western blotting analysis of the Nrf2 and HO-1 expression level (C: Control, L: LPS, T: LPS + TFNAs, CR: LPS + Cur, CT: LPS + Cur-TFNAs; β-actin was used as an internal control). (e) The relative protein expression intensity of Nrf2 and HO-1 (n = 4). Statistical analysis: *p < 0.05, **p < 0.01, and ***p < 0.001.