Figure 3.

MDCK cells transfected with AQP2-G215S displayed impaired transcellular osmotic water permeability. Cells were seeded onto 0.33 cm² polycarbonate filters, and replaced with fresh medium in the presence of 5 × 10⁻⁵ M indomethacin. Osmotic water transport was assayed with or without 5 × 10⁻⁵ M forskolin. The osmotic water transport (Pf) of AQP2-G215S-transfected and AQP2-WT-transfected cells was calculated as described in Material and Methods. WT, wild type; FK, Froskolin. Data were shown as mean ± SEM. n = 3, **p < 0.01, ***p < 0.001.