Table 6.
Intra-group variability in secretion of cytokines involved in Th1, Th2, Th9, and Th17 signaling from initiated colon tissue from tumor-free and tumor-bearing Apc+/Min-FCCC mice.
| Cytokine | Median Concentration (pg/ml/mg) | MAD* Expression | Proportion of mice with outlying expression† | |||
|---|---|---|---|---|---|---|
| Tumor Free | Tumor Bearing | Tumor Free | Tumor Bearing | Tumor Free | Tumor Bearing | |
| GM-CSF | 30.4 | 24.2 | 12.7 | 19.0 | 15% (2/13) | 15% (2/13) |
| Gro alpha | 941 | 566 | 683 | 358 | 15% (2/13) | 15% (2/13) |
| IFN gamma | 0.63 | 0.33 | 0.64 | 0.23 | 15% (2/13) | 15% (2/13) |
| IL-1 beta | 2.56 | 2.00 | 1.41 | 1.52 | 23% (3/13) | 0% (0/13) |
| IL-2 | 1.58 | 0.98 | 1.34 | 0.45 | 8% (1/13) | 8% (1/13) |
| IL-4 | 1.77 | 0.74 | 1.64 | 0.69 | 8% (1/13) | 15% (2/13) |
| IL-5 | 0.41 | 0.34 | 0.23 | 0.10 | 15% (2/13) | 15% (2/13) |
| IL-6 | 0.39 | 0.31 | 0.41 | 0.22 | 8% (1/13) | 0% (0/13) |
| IL-9 | 562 | 428 | 313 | 201 | 8% (1/13) | 15% (2/13) |
| IL-10 | 1.08 | 0.91 | 0.34 | 0.32 | 23% (3/13) | 15% (2/13) |
| IL-12p70 | 3.08 | 2.14 | 2.30 | 1.51 | 23% (3/13) | 23% (3/13) |
| IL-13 | 53.0 | 26.6 | 41.0 | 18.0 | 15% (2/13) | 23% (3/13) |
| IL-17a | 1.49 | 1.16 | 0.82 | 0.72 | 15% (2/13) | 0% (0/13) |
| IL-18 | 0.24 | 0.16 | 0.13 | 0.07 | 15% (2/13) | 8% (1/13) |
| IL-22 | 6.74 | 2.96 | 8.91 | 3.09 | 15% (2/13) | 15% (2/13) |
| IL-23 | 905 | 410 | 1313 | 374 | 8% (1/13) | 15% (2/13) |
| IL-27 | 10.8 | 9.68 | 2.46 | 2.69 | 8% (1/13) | 8% (1/13) |
| TNF alpha | 10.2 | 5.73 | 9.30 | 4.06 | 23% (3/13) | 23% (3/13) |
| Total mice | 31% (4/13) | 31% (4/13) | ||||
*Median absolute deviation; †Number of mice with cytokine expression values >3rd quartile + 1.5x interquartile range; in some mice, more than one cytokine was an outlier.