Figure 6.

The HGF-binding site for MET does not overlap with the HGF-binding sites for NEPH1 and NEPHRIN.A, schematic for the competitive ELISA. HGF was immobilized on the wells of an ELISA plate, and its binding to NEPH1, NEPHRIN, and MET alone and in combination with NEPH1 peptide-1 and NEPHRIN peptide-1 was analyzed. B, quantification of the data. All comparisons are with NEPH1 or NEPHRIN alone. Data are presented as mean ± SEM, and p-values were calculated using a two-tailed Student’s t test. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p < 0.0001.